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Results of the plasmid curing experiment with A. pleuropneumoniae AP76 transformants grown in PPLO broth in the absence (a, b) and presence (c, d) of sucrose; transformants were plated on PPLO agar without (a, c) and with kanamcyin selection (b, d). The solid symbols represent the arithmetic means of four independent experiments, with the bars documenting the standard deviations.
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